Metagenomics of Jacarepagua Lagoon

The Jacarepagua lagoon is a coastal shallow water body in the Rio de Janeiro city (Brazil) historically contaminated by Microcystins (\"MCs\" - toxin produced by cyanobacteria with carcinogenic potential for mammals). These molecules are stable and resistant to variation in some physical and chemical factors (i.e. temperature, pH) and some common proteases. Besides, many studies have evidenced the process of bioaccumulation of MCs in different organisms, its adsorption to sediment particles and also its biodegradation by microorganisms from the environment. Previously, we evaluated the removal of MC from water by microorganism assemblages and biodegradation process was observed in the sediment as well as in the interstitial water. Furthermore, we analysed the microbial community through sequencing 16S rDNA by Illumina and showed the differences between these two microbiomes. Moreover, major identified phyla were Chloroflexi, Proteobacteria, Firmicutes, OP3 and the identified OTUs several genera described as MC-degraders were detected

雅卡雷帕瓜泻湖（Jacarepagua lagoon）是巴西里约热内卢市境内的沿岸浅水环境，历史上曾受到微囊藻毒素（Microcystins，简称MCs）的污染——该毒素是蓝藻产生的、对哺乳动物具有致癌潜力的毒素。这类分子性质稳定，可耐受部分物理化学因素（如温度、pH值）以及常见蛋白酶的作用。此外，多项研究已证实微囊藻毒素可在多种生物体内发生生物富集、吸附于沉积物颗粒，并可被环境中的微生物降解。此前本团队已探究了微生物群落对水体中微囊藻毒素的去除效果，并在沉积物及间隙水中均观测到了微囊藻毒素的生物降解过程。此外，本团队通过Illumina平台对16S rDNA进行测序，分析了沉积物与间隙水的微生物群落组成，揭示了二者菌群结构的差异。本次研究共鉴定出的优势菌门包括绿弯菌门（Chloroflexi）、变形菌门（Proteobacteria）、厚壁菌门（Firmicutes）及OP3菌门；同时在检出的操作分类单元（Operational Taxonomic Units，OTUs）中，发现了多个已被报道具有微囊藻毒素降解能力的菌属。