Analysis of virome by high-throughput sequencing disclosed multiple infection in a single grapevine plant

ABSTRACT: Among phytosanitary problems of the grapevine, viruses stand out for their capacity of reducing the quality and yield of grapes. However, detecting and identifying viral infections in grapevines can be challenging. This study performed a high throughput sequencing (HTS) of the viral pathogens present in a vine showing virus-like symptoms to elucidate the etiology. HTS analysis reported in a hybrid grapevine with mild curling down of leaf edges, the presence of four viruses and viroids, which were probably implicated in the observed symptoms. The determined complete genomes showed high genetic identities with previously characterized isolates of homologous pathogens.

摘要：在葡萄植株的各类植物检疫问题中，病毒因能够降低葡萄的品质与产量而尤为突出。然而，检测并鉴定葡萄植株中的病毒感染仍颇具挑战。本研究针对表现出病毒样症状的葡萄植株开展了病毒病原体的高通量测序（High Throughput Sequencing, HTS），以阐明其病原学机制。通过对一株出现叶片边缘轻度下卷症状的杂交葡萄植株进行HTS分析，共检出4种病毒及类病毒，推测其与该植株的观测症状存在关联。本研究测得的完整基因组序列，与已完成特征鉴定的同源病原体分离株具有较高的遗传同一性。