Chr.genome.fa

Takifugu bimaculatus is an endemic marine fish in East Asia that belongs to Tetraodontidae in Tetraodontiformes. As a concentrated breeding fish species in Southeast coast of china, T. bimaculatus not only has high nutritional value and tasty flesh, but also has potential production capacity of tetrodotoxin (TTX).
Here we report a chromosome-level reference genome of T. bimaculatus generated employing the PacBio single molecule sequencing technique (SMRT) and High-through chromosome conformation capture (Hi-C) technologies. The genome sequences were assembled into 2,193 contigs with a total length of 404.21 Mb and a contig N50 length of 1.31 Mb. After chromosome-level scaffolding, 24 scaffolds were constructed with a total length of 371.68 Mb (91.92% of the total length of all contigs). Genome annotation identified 21,117 protein-coding genes, 3,471 ncRNAs. The high accurate, chromosome-level reference genome of T. bimaculatus provides the essential genome resource, not only for genome-scale selective breeding and comparative genome analysis of T. bimaculatus, but also for exploring the genetic mechanism of temperature adaptability evolution of takifugu.