Horizontal Gene Transfer of SGI-4 (from BBS 1356) or a plasmid containing<i> oriT</i><sub>SGI-4_966</sub> (from BBS 1636) to <i>Salmonella</i> serovars or other Enterobacteriaceae
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Conjugation efficiency of <em>Salmonella</em> Genomic Island 4 (SGI-4) from donor <em>Salmonella enterica</em> serovar I 4,[5],12:i:- strain BBS 1356 or a plasmid containing<em> oriT</em><sub>SGI-4_966</sub> from donor <em>Salmonella enterica</em> serovar I 4,[5],12:i:- strain BBS 1636 to recipient strains of <em>Salmonella enterica</em> serovars (Choleraesuis, Infantis, Enteritidis, and Senftenberg) or other Enterobacteriaceae (<em>Escherichia coli</em> and <em>Citrobacter youngae</em>)<em>.</em> The <em>oriT</em><sub>SGI-4_966</sub> nucleotide sequence spans nucleotides 4704810-4705775 of the <em>Salmonella enterica</em> serovar I 4,[5],12:i:- strain USDA15WA-1 genome (CP040686). Conjugation efficiency was calculated by dividing the colony forming units (CFU)/mL of transconjugants divided by the CFU/mL of donors. A transconjugant is a bacterial recipient that received DNA from the donor strain due to horizontal gene transfer.
本数据集针对两类供体材料的接合转移效率进行测定：其一为肠炎沙门氏菌（*Salmonella enterica*）血清型I 4,[5],12:i:-菌株BBS 1356携带的沙门氏菌基因组岛4（Salmonella Genomic Island 4, SGI-4）；其二为肠炎沙门氏菌血清型I 4,[5],12:i:-菌株BBS 1636携带的携带有SGI-4_966型转移起始位点（origin of transfer, oriT<sub>SGI-4_966</sub>）的质粒。上述供体材料的受体菌株包括肠炎沙门氏菌的猪霍乱血清型（Choleraesuis）、婴儿血清型（Infantis）、肠炎血清型（Enteritidis）、森夫滕贝格血清型（Senftenberg），以及肠杆菌科（Enterobacteriaceae）的大肠杆菌（*Escherichia coli*）与杨氏柠檬酸杆菌（*Citrobacter youngae*）。 SGI-4_966型转移起始位点的核苷酸序列覆盖肠炎沙门氏菌血清型I 4,[5],12:i:-菌株USDA15WA-1基因组（登录号CP040686）的4704810至4705775号核苷酸区域。 接合转移效率通过接合子每毫升菌落形成单位（colony forming units, CFU）数除以供体菌株每毫升菌落形成单位数计算得到。其中，接合子（transconjugant）指通过水平基因转移（horizontal gene transfer）从供体菌株获取外源DNA的细菌受体。
