Root Carbon and Nitrogen: Long-Term Nitrogen Deposition: Population, Community, and Ecosystem Consequences

The purpose of this experiment is to measure how adding nitrogen over a long time will affect the number of species, the type of species present, the amount of annual growth, and the change from year to year in the growth of each species in a plant community which is also relieved of grazing by large and small mammals. The experiment is being conducted within fields (A, B, C, and D) which were initially low in soil nutrients. There are 8 different levels of nitrogen addition with other nutrients added to ensure that nitrogen remains the limiting nutrient, and a control which receives no nutrients. There are 6 replicates of the 9 treatments in fields A, B, and C and 5 replicates in field D. The treatments were randomly assigned to the plots. In fields A, B, and C the plots are in 6 by 9 grids and are 4 by 4 meters in size with 1 meter aisles between plots. In field D the plots are 1.5 by 4 meters and are placed in a 3 by 17 grid. The plots are enclosed by a fence to keep out mammalian herbivores. Gophers are trapped and removed as they appear. Nitrogenfertilizer (NH4NO3) is applied twice per year, once in early May and once in late June. This experiment was begun in 1982 by David Tilman.

本实验旨在探究长期施加氮肥对植物群落的物种丰富度、现存物种组成、年生长量，以及各物种生长的年际动态变化的影响，同时该植物群落已排除大、小型哺乳动物的采食干扰。实验设置于初始土壤养分含量较低的地块（A、B、C、D）中，共设置8个氮肥施加梯度，同时配施其他养分以确保氮为唯一限制性因子，另设不施加任何养分的对照组。A、B、C三块地块的9种处理各设置6次重复，D地块的9种处理设置5次重复，所有处理组均随机分配至各试验样地。A、B、C地块的样地采用6×9的网格布局，单块样地面积为4米×4米，样地间设置1米宽的步道；D地块的样地面积为1.5米×4米，采用3×17的网格布局。所有样地均加装围栏以阻挡哺乳动物植食动物，出现的囊鼠均通过诱捕予以移除。氮肥（硝酸铵，NH4NO3）每年施加两次，分别于5月初与6月末进行。本实验由David Tilman于1982年启动。