Phylogenomic insights into the tribal-level phylogeny of Typhlocybinae

Typhlocybinae is one of the most diverse groups of leafhoppers, constituting an important component of phytophagous insect diversity. The traditional tribal-level phylogenetic relationships within this subfamily remain contentious, with differing hypotheses implying distinct evolutionary histories. This study contributes to resolving these controversies using phylogenomics. We newly sequenced low-coverage whole genomes for 54 species spanning the six classic tribes of Typhlocybinae. From these data, we extracted thousands of universal single-copy orthologs (USCOs) and ultraconserved elements (UCEs). Robust tribal-level phylogenies were reconstructed using multiple dataset matrices (USCO50, USCO70, USCO90, USCO_fna, ClipKIT_USCO70, UCE_fna) and tree-building strategies, including partitioned maximum likelihood with homogeneous models, unpartitioned heterogeneous mixture models, and the multi-species coalescent model. A particular focus was placed on elucidating the complex taxonomic stat..., , # Phylogenomic insights into the tribal-level phylogeny of Typhlocybinae

This README.txt file was generated on 2026-01-27 by Weiwei Ran

## File overview

To ensure universal readability, we have provided both .docx and .pdf formats for the file containing the same content (i.e., Supplementary_Material). This Supplementary_Material file includes all the information necessary to support the research findings, including the underlying data (Table S1) used to substantiate the conclusions. Additionally, we have provided separate charts for the Supplementary_Material.

File List:

**Table_S1.csv** Data statistics for phylogenomic studies

Definition for column headers:
Subfamily: A taxonomic rank above the level of Tribe.
Tribe: A taxonomic rank above the level of Genus.
Species: Taxonomic units at the species level.
Type of data: Indicates whether genomic or transcriptomic data were used.
Source: The provider of the data.
Sampling site: Geographic location...,