Transcriptomic analysis of SLAN+ vs SLAN- non-classical monocytes

Monocytes can be classified into four different subsets: classic, intermediate and SLAN- and SLAN+ non-classical monocytes. SLAN+ monocytes are significantly reduced in MM patients compare to the frequency found in healthy adults (HA). Thus, we sought to analyze the transcriptome of SLAN- and SLAN+ cells to unveil the differences among both populations We used microarrays to detail the global programme of gene expression underlying the differences among both cell subsets We developed a 12-color combination of mAbs to evaluate the contribution of each individual marker for identifying and subsetting BM TAMs in patients with MM (N = 5): CD36-UV405, CD16-UV525, CD163-BV450, CD45-BV525, CD206-BV610, CD86-BV660, CD62L-BV763, cyCD68-FITC, SLAN-PE, HLADR-PerCPCy5.5, CD300e-APC, CD14-APCH7. This panel allowed us to identify the aforementioned four monocyte subsets. Intermediate and non-classical monocytes from healthy donors were FACS sorted based on the 7-color mAb combination - HLADR-PacB, CD45-OC515, CD36-FITC, SLAN-PE, CD16-PECy7, CD300e-APC, CD14APCH7 - Gene expression data from CD36+ SLAN- monocytes, CD36- SLAN- monocytes, and CD36- SLAN+ monocytes.