Additional file 14 of H4-methylation regulators mediated epitranscriptome patterns and tumor microenvironment infiltration characterization in hepatocellular carcinoma

Additional file 14. Table S1. Basic information of hepatocellular carcinoma (HCC) datasets and external immunotherapy datasets included in this study. Table S2. Source and function of 36 histone H4 methylation (H4M) related regulators. Table S3. Primer sequences for qPCR. Table S4. The siRNA duplex sequences for functional experiments. Table S5. Microenvironment subtypes of HCC patients in TCGA. Table S6. Immune subtypes of HCC patients in TCGA. Table S7. TP53 mutation status of HCC patients in TCGA. Table S8. Intratumor heterogeneity score, proliferation score and Homologous Recombination Defects (HRD) score of HCC patients in TCGA-LIHC cohort. Table S9. UniCox regression and KM analyses for 36 H4M regulators. Table S10. Correlation analysis for 36 H4M regulators in meta-cohort. Table S11. Samples clustering in HCC RNA-seq meta cohorts. Table S12. Samples clustering in integrated external HCC cohorts. Table S13. Enrichment score of hallmark gene sets in HCC RNA-seq meta cohorts by GSVA analysis. Table S14. Enrichment score of HCC RNA-seq meta cohorts in cancer-related signatures. Table S15. Enrichment score of HCC RNA-seq meta cohorts in immune cell infiltration or immune signatures. Table S16. UniCox regression analysis for 133 hub genes. Table S17. The mRNA stemness index (mRNAsi) of HCC RNA-seq meta cohorts. Table S18. Immune response prediction by TIDE database. Table S19. Immunophenotype score predicted by TCIA database.

附加文件14：表S1 本研究纳入的肝细胞癌（hepatocellular carcinoma, HCC）数据集及外部免疫治疗数据集的基本信息。
表S2 36种组蛋白H4甲基化（histone H4 methylation, H4M）相关调控因子的来源与功能。
表S3 定量聚合酶链反应（quantitative polymerase chain reaction, qPCR）所用引物序列。
表S4 功能实验所用双链小干扰RNA（small interfering RNA, siRNA）序列。
表S5 TCGA队列中肝细胞癌患者的肿瘤微环境亚型。
表S6 TCGA队列中肝细胞癌患者的免疫亚型。
表S7 TCGA队列中肝细胞癌患者的TP53突变状态。
表S8 TCGA-LIHC队列中肝细胞癌患者的肿瘤内异质性评分、增殖评分及同源重组缺陷（homologous recombination defects, HRD）评分。
表S9 36种H4M相关调控因子的单因素Cox回归与Kaplan-Meier（KM）分析结果。
表S10 荟萃队列中36种H4M相关调控因子的相关性分析结果。
表S11 肝细胞癌RNA测序荟萃队列的样本聚类结果。
表S12 整合外部肝细胞癌队列的样本聚类结果。
表S13 基于基因集变异分析（gene set variation analysis, GSVA）得到的肝细胞癌RNA测序荟萃队列中特征基因集的富集得分。
表S14 肝细胞癌RNA测序荟萃队列在癌症相关特征中的富集得分。
表S15 肝细胞癌RNA测序荟萃队列在免疫细胞浸润或免疫特征中的富集得分。
表S16 133个核心基因的单因素Cox回归分析结果。
表S17 肝细胞癌RNA测序荟萃队列的mRNA干细胞指数（mRNA stemness index, mRNAsi）。
表S18 基于TIDE数据库的免疫应答预测结果。
表S19 基于TCIA数据库预测的免疫表型评分。