SH1039816.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE为界定、识别、交流以及处理基于DNA的物种假说（DNA-based Species Hypotheses，SH）提供了统一的方法框架。研究人员通过应用一系列动态距离阈值（0.5%~3.0%），将国际核苷酸序列数据库中的所有真菌ITS序列聚类至近似物种水平。所有物种假说均会被赋予唯一且稳定的数字对象唯一标识符（DOI）格式的名称，其分类学与生态学注释将通过分布式网络第三方注释工作进行核验。每个物种假说都会尽可能结合其包含的所有序列的鉴定结果，关联至对应的分类单元名称及其分类层级（如门、纲、目等）。研究人员将选取一条自动或人工指定的序列，作为该物种假说的代表序列。上述代表序列已公开发布于（https://unite.ut.ee/repository.php），可供科研社群用于本地序列相似性搜索、新一代测序分析流程等场景。随着公共真菌ITS序列库的规模不断增长，该系统与配套数据将自动完成更新。