RNA-Seq Profiling of Intestinal Expression of Xenobiotic Processing Genes in Germ-Free Mice

This study aimed to quantify and compare the mRNA abundance of all major XPGs in liver and intestine between conventional (CV) and germ-free (GF) mice using RNA-Seq. The CV RNA-Seq data were previously uploaded to GEO database by the same research group (GSE79848). All CV and GF mice were age-matched, and were analyzed under the same conditions (diet, water, bedding, and animal facility). Overall design: mRNA profiles of liver and four sections of intestine of conventional (CV) and germ-free (GF) mice were generated by RNA-Seq, in triplicate, by illumina HiSeq 2000.