Data from: Development of nuclear microsatellite markers for the Japanese conifers Tsuga diversifolia and T. sieboldii (Pinaceae)

Nuclear microsatellite markers were developed for the two Tsuga species native to the Japanese Archipelago, Tsuga diversifolia and T. sieboldii, and a population with genetic affinities to T. diversifolia on Ulleung Island, Korea. Tsuga diversifolia and T. sieboldii are widespread dominant trees of temperate and subalpine forests in Japan but to date no genetic markers have been developed for these species. Fifteen polymorphic loci were developed and characterized, of which 14 are reliably amplified in each taxon. Across both species and the Ulleung Island population, the number of alleles per locus ranged from 3 to 26 (average = 13.93) and observed heterozygosity ranged from 0.005 to 0.935 (average = 0.535). In addition, all 15 loci were successfully amplified in a single accession of the Chinese species, T. chinensis. These markers will be useful for investigating the species’ biogeography, range-wide genetic diversity, conservation genetic issues and potential for hybridisation.

本研究为日本列岛原生的两种铁杉属物种——日本铁杉（Tsuga diversifolia）和锡尔博德铁杉（Tsuga sieboldii），以及韩国郁陵岛上与日本铁杉具有遗传亲缘关系的种群，开发了核微卫星标记（nuclear microsatellite markers）。日本铁杉与锡尔博德铁杉是日本温带及亚高山森林中广泛分布的优势树种，但截至目前尚未针对这两个物种开发出遗传标记。本研究开发并鉴定了15个多态性位点，其中14个可在每个类群中稳定扩增。在两个物种及郁陵岛种群中，每个位点的等位基因数范围为3至26（平均值=13.93），观测杂合度范围为0.005至0.935（平均值=0.535）。此外，所有15个位点均可在一份中国铁杉（Tsuga chinensis）的单份样品中成功扩增。这些标记将有助于研究该类群的生物地理学、分布区范围内的遗传多样性、保护遗传学问题以及杂交潜力。