Mass spectroscopy analysis of Nuclear YAP1 interacting proteins

YAP1 regulates the expression of several genes involved in diverse cell biology, including cell growth, cell survival, cell differentiation, cell transformation, carcinogenesis, and metastasis. In the present study, we employed co-immunoprecipitations combined with mass spectroscopy to identify nuclear YAP1 interacting proteins in the well-established human prostate tumor cell models steroid. Our proteomics analysis demonstrated that androgen hormone signaling regulates the YAP1 interacting proteins. Our functional annotation clustering showed that YAP1-associated proteins were enriched for diverse cellular pathways, including RNA transport, RNA processing, RNA translation, posttranscriptional regulation, protein folding, metabolism, etc. Furthermore, we characterized the molecular and functional interaction between multifunctional, polycomb group protein SCML2 and YAP1 in cultures. Our results demonstrate that YAP1 cooperates with the epigenetic regulatory protein SCML2 to module cell growth, cell survival, and oncogenesis downstream of steroid hormone androgen signaling.