Crystal structure and anti-breast cancer activity evaluation of a nanosized bismuth(V)-containing coordination complex based on the F-decorated ligand

In the current study, a new heteroleptic triorganobismuth (V) complex with the chemical formula of Bi(phenyl)₃(2,3-dfa)₂ has been successfully prepared by using C donor phenyl ring and O donor 2,3-difluorobenzoic acid (2,3-H₂dfa) ligands, which has been fully characterized by single crystal X‐ray crystallography, elemental analysis, powder X-ray diffraction along with the thermogravimetric analysis. Furthermore, a green hand grinding technique was used to get the nanocrystals of <b>1</b> with an average size of 200 nm as revealed via the scanning electron microscopy (SEM). The inhibitory effect of BT474 cells proliferation after nano <b>1</b> treatment was detected with CCK-8 assay. The apoptosis of BT474 cells was measured with FITC-Annexin V/PI double staining assay, the influence on ROS accumulation was determined with DCFH-DA staining.

本研究成功制备了一种新型杂配型三有机铋(V)配合物（heteroleptic triorganobismuth(V) complex），其化学式为Bi(苯基)₃(2,3-二氟苯甲酸根)₂，所用配体为碳给体苯基配体与氧给体2,3-二氟苯甲酸（2,3-H₂dfa）配体。该配合物通过单晶X射线衍射（single crystal X-ray crystallography）、元素分析（elemental analysis）、粉末X射线衍射（powder X-ray diffraction）以及热重分析（thermogravimetric analysis）完成了全面表征。此外，本研究采用绿色手工研磨技术制备了编号为1的目标产物纳米晶体，经扫描电子显微镜（scanning electron microscopy, SEM）检测可知其平均粒径为200 nm。采用CCK-8实验法检测了纳米1处理后BT474细胞的增殖抑制效果；通过FITC-Annexin V/PI双染色法测定了BT474细胞的凋亡情况；并利用DCFH-DA染色法检测了其对活性氧（reactive oxygen species, ROS）积累的影响。