Additional file 1 of CCR2 macrophage response determines the functional outcome following cardiomyocyte transplantation

Additional file 1: Supplement figures. Figure S1. Representative gating strategy for identifying the cardiac immune cells using flow cytometry and sorting them for single cell RNA sequencing. Figure S2. Single-cell RNA sequencing reveals the differentially expressed genes of the various cardiac immune cell clusters between C57BL/6J and Rag2del mice after MI. Figure S3. Assessment of cardiac ventricular remodelling. Figure S4. Differentially expressed transcripts in the heart and blood. Figure S5. GO terms for the DE transcripts in the heart and blood between Rag2delMI and Rag2delMI-CM groups. Figure S6. The most significant transcripts obtained using machine learning feature selection.

附加文件1：补充图表。图S1：利用流式细胞术（flow cytometry）鉴定心脏免疫细胞并分选以进行单细胞RNA测序（single cell RNA sequencing）的代表性设门策略。图S2：单细胞RNA测序揭示心肌梗死后C57BL/6J小鼠与Rag2del小鼠各心脏免疫细胞簇的差异表达基因。图S3：心脏心室重构评估。图S4：心脏与血液中的差异表达转录本（differentially expressed transcripts）。图S5：Rag2delMI组与Rag2delMI-CM组心脏及血液中差异表达转录本的基因本体（Gene Ontology，GO）条目。图S6：通过机器学习特征筛选获得的最显著转录本。