Gene expression during conidia germination in Tolypocladium ophioglossoides

Comparative transcriptomics to assess infectiou process of Tolypocladium ophoiglossoides, a parasite of false truffle mushrooms. Two media, PDA and mushroom powder agar were used, and four stages of conidial germination were examined. Conidia germination is critical for fungi to colonize various habitats. We sampled RNA expression at four stages of conidia germination, including fresh conidia (15min), polar growth (120min), doubling of long axis (240min), and first hyphal branching (360min) in Tolypocladium ophioglossoides. Cultures were made on PDA and mushroom powder medium, and two biological replicates were collected for all data points. The growth was under a labratory condition of 25C and constant light.