Additional file 1 of Temporal responses of conserved miRNAs to drought and their associations with drought tolerance and productivity in rice

Additional file 1: Table S1. Agronomic traits measure among six rice genotypes in drought-treated and well-watered fields. * and ** indicate significant differences at p < 0.05 and p < 0.01 by independent t test between samples from drought and well-watered fields. NA is short for “not available”. Table S2. Content of H2O2 (mmol/g FW) measured among six rice genotypes in drought and well-watered fields. * indicates significant differences at p < 0.05 by independent t test between samples from drought and well-watered fields. Table S3. Osmotic potential measured among six rice genotypes in drought and well-watered fields. * and ** indicate significant differences at p < 0.05 and p < 0.01 by independent t test between samples from drought and well-watered fields. Table S4. Total anti-oxidant capacity (U/g FW) measured among six rice genotypes in drought and well-watered fields. * and ** indicate significant differences at p < 0.05 and p < 0.01 by independent t test between samples from drought and well-watered fields. Table S5. Basic information for sequenced samples. Table S6. Number of expressed, available, drought-responsive miRNAs (DRMs), and recovery-related miRNAs (RRMs). Table S7. Validation of expression patterns and correlation-based predictions by former functionally characterized miRNAs in rice. Table S8. Number of recovery-related miRNAs in five regulation patterns from timepoint D5 to recovery stage (R). Table S9. Number of recovery-related miRNAs (RRMs) in five regulation patterns from timepoint D5 to recovery stage (R). “Varied” indicates the regulation of a miRNA varies among genotypes. Table S10. Target genes predicted by TargetFinder and psRobot for drought-responsive miRNAs. GDP is short for growth, development, and reproduction. DT is short for drought tolerance. NA is short for not assigned. Table S11. Number of predicted target genes of differentially expressed miRNAs in drought responsive genes (DRGs) with different Pearson’s Correlation Coefficient (PCC) values. Table S12. Target genes predicted for miR408-3p and miR408-5p and their PPC values in correlations between miRNA and mRNA. Table S13. Pearson’s correlation coefficients (PCCs) of genes related to plant height (PH) and drought-tolerance (DT) with OsmiR1870-3p and OsmiR1870-5p. Table S14. Basic information for six rice genotypes. Table S15. Information for RNA-sequenced samples. Table S16. Primers of six miRNAs and the reference (U6) for qPCR. Table S17. List of GDP (growth, development, and reproduction)- and DT (drought-tolerance)-associated drought-responsive genes. These DT-associated genes have been functionally characterized. These GDP-associated genes are related to plant height, number of tillers, grain yield, and biomass by former studies.

附加文件1：表S1。六个水稻基因型在干旱处理与正常灌溉田块中的农艺性状测定结果。*和**分别代表通过独立样本t检验，干旱组与正常灌溉组样本间差异具有统计学显著性（p<0.05）与极显著性（p<0.01）。NA为"not available（无有效数据）"的缩写。
表S2。六个水稻基因型在干旱处理与正常灌溉田块中的过氧化氢（H₂O₂）含量（单位：mmol/g 鲜重（FW））测定结果。*代表通过独立样本t检验，干旱组与正常灌溉组样本间差异具有统计学显著性（p<0.05）。
表S3。六个水稻基因型在干旱处理与正常灌溉田块中的渗透势测定结果。*和**分别代表通过独立样本t检验，干旱组与正常灌溉组样本间差异具有统计学显著性（p<0.05）与极显著性（p<0.01）。
表S4。六个水稻基因型在干旱处理与正常灌溉田块中的总抗氧化能力（单位：U/g 鲜重（FW））测定结果。*和**分别代表通过独立样本t检验，干旱组与正常灌溉组样本间差异具有统计学显著性（p<0.05）与极显著性（p<0.01）。
表S5。测序样本基础信息。
表S6。表达型、有效型、干旱响应性微小RNA（drought-responsive miRNAs，简称DRMs）以及恢复相关微小RNA（recovery-related miRNAs，简称RRMs）的数量统计。
表S7。通过水稻中已功能验证的微小RNA，对表达模式及基于相关性的预测结果进行验证。
表S8。从时间点D5至恢复阶段（R）的五种调控模式下的恢复相关微小RNA（RRMs）数量统计。
表S9。从时间点D5至恢复阶段（R）的五种调控模式下的恢复相关微小RNA（RRMs）数量统计。"变异型（Varied）"代表某一微小RNA的调控模式在不同水稻基因型间存在差异。
表S10。通过TargetFinder和psRobot预测得到的干旱响应性微小RNA的靶基因。GDP为"生长、发育与繁殖（growth, development, and reproduction）"的缩写，DT为"耐旱性（drought tolerance）"的缩写，NA为"not assigned（未标注）"的缩写。
表S11。在不同皮尔逊相关系数（Pearson’s Correlation Coefficient，简称PCC）区间内，干旱响应基因（drought responsive genes，简称DRGs）中差异表达微小RNA的预测靶基因数量统计。
表S12。miR408-3p与miR408-5p的预测靶基因，及其在微小RNA与mRNA相关性分析中的皮尔逊相关系数（PCC）值。
表S13。与株高（plant height，简称PH）及耐旱性（DT）相关的基因，分别与OsmiR1870-3p和OsmiR1870-5p的皮尔逊相关系数（PCCs）。
表S14。六个水稻基因型的基础信息。
表S15。RNA测序样本的相关信息。
表S16。六个微小RNA以及实时定量聚合酶链反应（quantitative real-time PCR，简称qPCR）内参基因U6的引物信息。
表S17。与生长、发育与繁殖（GDP）及耐旱性（DT）相关的干旱响应基因列表。其中，耐旱性相关基因已完成功能验证；生长、发育与繁殖相关基因在既往研究中被证实与株高、分蘖数、籽粒产量及生物量相关。