Analysis of ��S-locus haplotypes in selected accessions.

aDNA gel blot analysis was used to determine whether each accession contains (+) or does not contain (?) sequences corresponding to: the extracellular domain (eSRK) and last exon (exon 7) of ��SRKA or ��SRKC, the ��SCR1 sequence, the extracellular domain of ��SRKB, and ��SCRB. Results shown in bold type were not reported previously and the corresponding DNA gel blot images are shown in Figure 1. Results shown in regular type were previously reported in [27].bPCR amplification was used to determine if the ��SC-associated deletion in the first intron of ARK3 was present (+) or absent (?).cPolymorphisms in the ARK3 gene, between exons two and five, rather than the first intron, have been reported [27] and these data support those shown here.dIn addition to the Kas-2 and C24 accessions, this study shows that the ��S locus of the Nok-3 accession also experienced inter-haplotypic recombination.