Spike times from intracellular responses to step currents in juvenile zebra finch caudal mesopallium

Data from intracellular recordings obtained from neurons in the caudal mesopallium of 52 juvenile (30-35 days post hatch) zebra finches of both sexes. All procedures were performed according to National Institutes of Health guidelines and protocols approved by the University of Virginia Institutional Animal Care and Use committee.Details of rearing conditions and data collection are given in the associated publication. The <b>spike_times.zip</b> archive contains files corresponding to one intracellular recording epoch from a single neuron. Filenames follow the format <b>{cell_id}_{epoch}.pprox</b>. Each epoch comprises 20 sweeps. Each sweep was 6 s in duration: 100 ms at 0 pA, a 2000-ms depolarizing step, 1000 ms at 0 pA, 500 ms at -20 pA, 500 ms at -40 pA, 500 ms at -20 pA, and 1400 ms at 0 pA. The amplitude of the depolarizing pulse was systematically varied within each epoch over a range that was set so that at least 10 sweeps in each epoch evoked action potentials without eliciting depolarization block. The range of current steps was adjusted throughout the recording as needed.The data are stored in a custom JSON-based format called pprox. Briefly, the JSON object contains metadata about the recording at the top level, and a field called "pprox" that comprises a list of objects corresponding to individual sweeps. Each sweep contains a field called "events" with a list of the times of the spikes that occurred during the trial, along with estimates of the membrane potential (Vm), series resistance (Rs), and input resistance (Rm).The Python code used to produce the pprox files from the raw intracellular recording data and to analyze the results is available on github.

本数据集源自对52只雌雄两性幼年斑胸草雀（孵化后30~35天）尾侧中脑皮质神经元的细胞内记录。所有实验操作均遵循美国国立卫生研究院（National Institutes of Health）指南，并经弗吉尼亚大学机构动物护理与使用委员会批准。饲养条件与数据采集的详细信息详见相关发表文献。**spike_times.zip** 压缩包内含单个神经元一次记录时段的对应文件，文件名格式为**{cell_id}_{epoch}.pprox**。每个记录时段包含20次扫描，单次扫描时长为6秒，时序如下：100毫秒处于0皮安培（pA）电流下，随后为2000毫秒去极化阶跃，接着依次为1000毫秒0皮安培、500毫秒-20皮安培、500毫秒-40皮安培、500毫秒-20皮安培，最后为1400毫秒0皮安培。每个记录时段内的去极化脉冲幅度会进行系统性调整，调整范围设置为确保该时段内至少有10次扫描可诱发动作电位且不引发去极化阻滞；记录过程中可根据实际需求调整电流阶跃的范围。数据以自定义的基于JSON的pprox格式存储。简言之，顶层JSON对象包含本次记录的元数据，另有一个名为"pprox"的字段，其内容为对应各次扫描的对象列表。每次扫描均包含一个名为"events"的字段，该字段存储了本次扫描期间记录到的锋电位时刻列表，同时附带膜电位（Vm）、串联电阻（Rs）与输入电阻（Rm）的估算值。用于从原始细胞内记录数据生成pprox格式文件并分析实验结果的Python代码已开源至GitHub平台。