Ip, Wallace (2010) CIL:6263, Oryctolagus cuniculus, skeletal muscle cell. CIL. Dataset

Rabbit psoas skeletal muscle fibers were blended in the presence of 5 mM MgATP to dissociate thick and thin filaments. The medium was then gently replaced with 0.3M KCl to dissociate thick filaments. Dissociated filaments were adsorbed onto mica flakes (Heuser JE, J Mol Biol. 1983 Sep 5;169(1):155-95), quick-frozen by contact with a liquid helium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 11 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev.

This image was recorded on Kodak 4489 film at 75,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner.

Stereo pair with CIL:6264.

将家兔的 psoas 骨骼肌纤维在 5 mM MgATP 的存在下混合，以解离粗细肌丝。随后，通过将介质轻轻替换为 0.3M KCl 来解离粗肌丝。解离后的肌丝被吸附于云母片（Heuser JE，J Mol Biol. 1983 Sep 5;169(1):155-95）上，随后在 Heuser 型冷冻压机上通过接触液氦冷却的铜块进行快速冷冻，并在 Balzers 400 冷冻断裂机上进行冷冻蚀刻。样品随后以 11 度的转速进行 Pt-C 复制，并在操作于 100 kev 的 JEOL 100CX2 透射电子显微镜下进行可视化。该图像是在 Kodak 4489 胶片上以 75,000 倍放大倍数记录的。在 +5 度和 -5 度处拍摄了立体对。胶片使用 Nikon Coolscan 9000ED 扫描仪进行扫描，像素间距为 20 微米。与 CIL:6264 对应的立体对。