File S1 - Exendin-4 Reduces Ischemic Brain Injury in Normal and Aged Type 2 Diabetic Mice and Promotes Microglial M2 Polarization

Contains the following files: Figure S1. The HFD regime leads to increased body weight gain and increased fasting and fed blood glucose levels. Body weight in 2-month-old healthy mice or after 12 months of HFD (A). Fasting blood glucose levels in 12-month-old HFD-fed and 2-month-old healthy mice are shown (B). Fed blood glucose levels in HFD-fed mice (14 months old) and in 2-month-old healthy mice at the time of the MCAO surgery (C). Data are presented as means ± SEM. **** denotes p<0.0001. Figure S2. Ex-4 treatment does not change inflammatory markers in microglia cultures. Microglial cell cultures were stimulated with the pro-inflammatory mediator LPS (10 ng/ml) for 24 h with or without Ex-4 (40 ng/ml). IL-1β (A) and MCP-1 (B) were measured in the microglia media, Graphs represent data from 3 independent experiments. Data are presented as means ± SEM. ***p<0.001.
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