Expression data from homozygous deletion of the Lgi1 gene in murine neural precursor-like cells. Mus musculus

The hypercellularity in the outer layers of the cortex in Lgi1 null mice suggested that Lgi1 possibly plays a role in controlling cell migration dynamics. To investigate this hypothesis, we first generated and immortalized neural precursor-like cells (NPC), isolated from Lgi1 null and wild type mice at E13.5 stage using Large-T antigen. Subsequently, we compared gene expression patterns between the immortalized NPC-like cells with the two different Lgi1 genotypes using Affymetrix GeneChip Mouse Gene 1.0 ST Array. Our studies suggest that Lgi1 has a role in regulating neuronal migration and their synapse formation and that its absence/dysregulation can result in seizure despite a histologically subtle cortical dysplastic phenotype. Overall design: RNAs were extracted from the null and wild type NPC cells, respectively. The samples were biologically triplicated. RNA samples were hybridized to Mouse Gene 1.0 ST Array.