Screening for simple sequences repeat markers in Hemileia vastatrix

Screening of potential microsatellite loci was performed using a modified M13-tailed primer method (Schuelke, 2000), as previously described (Díaz-Valderrama & Aime, 2016; Koch & Aime, 2018). We accomplished an initial screening of ~100 microsatellite markers (Table S2) located on separate scaffolds on a set of six H. vastatrix specimens collected from three geographical regions (Puerto Rico, Jamaica, and Cameroon).

本研究采用改良的M13尾引物法（M13-tailed primer method，Schuelke, 2000）开展潜在微卫星位点（microsatellite loci）的筛选，具体操作参照已发表文献（Díaz-Valderrama & Aime, 2016; Koch & Aime, 2018）。本研究对位于不同基因组支架（scaffold）上的约100个微卫星标记（Table S2）进行了初步筛选，所用标本为采自波多黎各、牙买加、喀麦隆三个地理区域的6份H. vastatrix样本。