Putative indicators of thyroid hormone system disruption - a transcriptional footprint of developmental hypothyroidism in hippocampus

Thyroid hormone (TH) system disrupting compounds can impair brain development by perturbing TH action during critical life stages. Human exposure to TH system disrupting chemicals is therefore of great concern. To better protect humans against such chemicals, sensitive test methods that can detect effects on the developing brain are critical. Worryingly, however, current test methods are not sensitive and specific towards TH-mediated effects. To address this shortcoming, we performed Bulk-RNA-sequencing of rat brains developmentally exposed to two different thyroperoxidase (TPO) inhibiting compounds, the medical drug methimazole (MMI) or the pesticide amitrole.甲状腺激素（TH）系统干扰化合物可通过在生命关键发育阶段扰乱甲状腺激素的生理作用，损害大脑发育。因此，人类暴露于甲状腺激素系统干扰化学物质的问题备受关注。为更好地保护人类免受此类化学物质的危害，开发能够检测对发育中大脑产生影响的灵敏检测方法至关重要。然而令人担忧的是，当前的检测方法对甲状腺激素介导的效应并不具备足够的灵敏性与特异性。为解决这一缺陷，我们对发育阶段暴露于两种不同甲状腺过氧化物酶（TPO）抑制剂的大鼠脑组织开展了批量RNA测序（Bulk-RNA-sequencing），这两种抑制剂分别为医用药物甲巯咪唑（MMI）与农药杀草强（amitrole）。