Quantitative analysis of tet inducible SOX18 during hematopoietic differentiation and Natural killer cell differentiation

Using inducible SOX18 human PSCs, we found overexpression of SOX18 showed change of transcriptional program at D8 especially T cell receptor and Toll like receptor signaling pathways. So this suggests SOX18 has an effect on lymphoid differentiation program. Specifically overexpressed SOX18 enhance NK cell production, and related to metabolism pathway. This finding that SOX18 promotes development of progenitors with superior NK cell potenital will advance our understanding of molecualr network regulating specification on lymphoid cells and can help to improve the scalability of NK CELL production form hPSCs form immunotherapies. mRNA profiling of 18 samples for 5 different populations obtained from hematopoietic differentiation cultures of hESCs with doxycyclin (DOX)-inducible SOX18 expression. Samples obtained form HE isolated on day 4 (D4) of diiferentiation following D2 through D4 DOX treatment, hematopoiteic progenitors isolated on D8 of differntiation following D2-8 DOX treatment, and NK cell generated after 3 weeks of cultures of hematopoietic progenitors following DOX2-8 treatment. Cultures without DOX treatment were used as a control.