Raw data package from: Subfunctionalization of Peroxisome Proliferator Response Elements Accounts for Retention of Duplicated fabp1 Genes in Zebrafish
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资源简介:
Raw data package contains the following:
1) File folder titled "ClustalOmega fabp1", this file folder contains:
a) PHYLIP text files (fabp1_Aminoacids_Clustal_PHYLIP.rtf, fabp1_mRNA_Clustal_PHYLIP.rtf, fabp1_Promoter_Clustal_PHYLIP.rtf) containing raw data for the multiple-sequence alignments and their associated draw tree files, and
b) a multi-fasta file containing the amino acid, mRNA, and promoter sequence files for Danio rerio (zebrafish) fabp1a, fabp1b.1, and fabp1b.2 and Lepisosteus oculatus (spotted gar) fabp1 genes.
2) A GraphPad Prism 6.0 file containing the curve fitting and analyses of data presented in the associated manuscript (Laprairie et al 2016 BMC Evol Biol Analyses.pzfx).
3) A Microsoft Excel files containing the raw data values for all data presented in the associated manuscript (Laprairie et al 2016 BMC Evol Biol Raw Data.xlsx).
4) The text output file for found peroxisome proliferator-activated receptor response elements (PPREs) in the isolated Danio rerio (zebrafish) fabp1a, fabp1b.1, and fabp1b.2 and Lepisosteus oculatus (spotted gar) fabp1 promoters described in the associated manuscript.
5) The multi-fasta file containing the sequenced pGL3 promoter constructs for Danio rerio (zebrafish) fabp1a, fabp1b.1, and fabp1b.2; fabp1a 5'flanking region mutant, fabp1a direct repeat 1 region mutant, fabp1b.1 5'flanking region mutant, fabp1b.1 direct repeat 1 region mutant; Lepisosteus oculatus (spotted gar) fabp1; fabp1 PPRE-1 mutant, fabp1 PPRE-2 mutant promoters sequences from the positive strand (RVprim3 primer) and negative strand (GLprim2 primer) of the pGL3 plasmid (Laprairie et al 2016 BMC Evol Biol Plasmid Sequencing.rtf).
本原始数据包包含以下内容:
1) 名为“ClustalOmega fabp1”的文件夹,该文件夹内包含:
a) 三份PHYLIP格式文本文件(fabp1_Aminoacids_Clustal_PHYLIP.rtf、fabp1_mRNA_Clustal_PHYLIP.rtf、fabp1_Promoter_Clustal_PHYLIP.rtf),存储多序列比对的原始数据及其配套的进化树绘制文件;
b) 一个多序列FASTA格式文件,包含斑马鱼(Danio rerio)fabp1a、fabp1b.1、fabp1b.2以及斑点雀鳝(Lepisosteus oculatus)fabp1基因的氨基酸序列、mRNA序列与启动子序列文件。
2) 一份GraphPad Prism 6.0格式文件(文件名为Laprairie et al 2016 BMC Evol Biol Analyses.pzfx),用于对关联论文(Laprairie等,2016年发表于《BMC Evolutionary Biology》)中的展示数据进行曲线拟合与分析。
3) 一份Microsoft Excel格式文件(文件名为Laprairie et al 2016 BMC Evol Biol Raw Data.xlsx),存储关联论文中所有展示数据的原始数值。
4) 一份文本输出文件,记录了关联论文中所描述的、在已分离的斑马鱼(Danio rerio)fabp1a、fabp1b.1、fabp1b.2及斑点雀鳝(Lepisosteus oculatus)fabp1基因启动子内发现的过氧化物酶体增殖物激活受体反应元件(peroxisome proliferator-activated receptor response elements,PPREs)的相关信息。
5) 一份多序列FASTA格式文件,包含测序得到的pGL3启动子构建体序列,具体涵盖:斑马鱼(Danio rerio)fabp1a、fabp1b.1、fabp1b.2;fabp1a 5'侧翼区突变体、fabp1a 直接重复1区突变体、fabp1b.1 5'侧翼区突变体、fabp1b.1 直接重复1区突变体;斑点雀鳝(Lepisosteus oculatus)fabp1;fabp1 PPRE-1突变体、fabp1 PPRE-2突变体的启动子序列,上述序列均取自pGL3质粒的正链(以RVprim3为引物)与负链(以GLprim2为引物),对应文件为Laprairie et al 2016 BMC Evol Biol Plasmid Sequencing.rtf。
创建时间:
2016-07-27



