Multispecies PCR amplyfication of CESTR bull blood samples from VUZV.CZ collection genome sequencing and assembly. Bos taurus

The historical breeds of farm animals, conserved in frame of the genetic resources programmes, are considered to be a source of utilizable allelic variants of disease resistance genes. In contrast to modern production breeds, the spectrum of alleles is supposed to reflect infection pressure specific for the geographic region and breeding conditions. Screening for the diversity of innate immunity receptors belonging to the Toll-like receptor (TLR) family has been carried out in two historical cattle breeds (Czech Red and Czech Red Pied). The advantages of the PacBio NGS platform were used for facilitated discovery of sequence polymorphism and haplotype structure in pooled amplicons from the coding regions of ten bovine TLRs.