Mean(LR.no) IAA

After 3 days of treatment, plates were scanned with CCD flatbed scanners (EPSON Perfection V600 Photo, Seiko Epson, Nagano, Japan), and images used to quantify root parameters with FIJI, by using the tool ‘segmented line’ (Schindelin et al., 2012) as described in Ristova and Busch (2017). In particular, we quantified: primary root length on day 10 (P), growth rate of P after treatment (P2), branching zone or the length of P between the first and last visible lateral root (R), average lateral root length (LRL), and visible lateral root number (LR.No).

处理3天后，采用CCD平板扫描仪（EPSON Perfection V600 Photo，精工爱普生公司，日本长野）对培养板进行扫描成像；随后参照Ristova与Busch（2017）的研究方法，借助FIJI软件中的「分割线（segmented line）」工具（Schindelin等，2012），对所得图像开展根系参数量化分析。具体量化参数包括：第10天的主根长度（P）、处理后主根的生长速率（P2）、分支区段长度（即主根上首个可见侧根与末次可见侧根之间的区段，记为R）、平均侧根长度（LRL）以及可见侧根数量（LR.No）。