Effects of some selected metal ions, inhibitors and chemical reagents on the purified FAL from Fusarium annulatum Bugnicourt strain CBS.

The enzyme assay was performed after pre-incubation of the enzyme with each tested chemical compound, for 1 h at 40°C. The non-treated and dialyzed enzyme was considered as 100% for the metal ion assay. The lipase activity measured in the absence of any inhibitor or reducing agent was taken as the control and considered as 100%. Residual activity was measured at pH 9 and 40°C, using TC8 as a substrate.