Gene expression profiling reveals cytoplasmic U1 snRNA accumulation may cause cancers

In this study, we built a model of cytoplasmic aggregation of U1 snRNA in a rat cell line. By comparing the gene expression profiling of U1 snRNA accumulated cells with their controls, 916 (about 4% of 20,000) genes were identified significantly differentially expressed. These 595 over-expressed genes and 321 repressed genes were further analyzed by GO and KEGG pathway enrichment. As a result, three of 12 enriched pathways are well-known cancer pathways, while nine of them were associated to cancers in previous studies. These findings suggest that cytoplasmic U1 snRNA accumulation may cause cancers.本研究在大鼠细胞系中构建了U1小核RNA（U1 snRNA）胞质聚集模型。通过对比U1小核RNA（U1 snRNA）富集细胞与其对照细胞的基因表达谱，共鉴定出916个（约占20000个基因的4%）显著差异表达基因。针对其中595个上调基因与321个下调基因，进一步开展基因本体（Gene Ontology，GO）和京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes，KEGG）通路富集分析。结果显示，12条富集通路中有3条为公认的癌症相关通路，另有9条在既往研究中被报道与癌症存在关联。上述研究结果表明，U1小核RNA（U1 snRNA）胞质聚集可能诱发癌症。