The results of RNA seq analysis: Curcumin promotes progression of AApoAII amyloidosis and peroxisome proliferation in mice by activating the PPARα signaling pathway

Curcumin is a polyphenol compound that exhibits multiple physiological activities. To elucidate the mechanisms by which curcumin affects systemic amyloidosis, we investigated amyloid deposition and molecular changes in a mouse model of amyloid apolipoprotein A-II (AApoAII) amyloidosis, in which mice were fed a curcumin-supplemented diet. Curcumin supplementation for 12 weeks significantly increased AApoAII amyloid deposition relative to controls, especially in the liver and spleen. Liver weights and plasma ApoA-II and high-density lipoprotein concentrations were significantly elevated in curcumin-supplemented groups. RNA-sequence analysis revealed that curcumin intake affected hepatic lipid metabolism via the peroxisome proliferator-activated receptor (PPAR) pathway, especially PPARα activation, resulting in increased Apoa2 mRNA expression. The increase in liver weights was due to activation of PPARα and peroxisome proliferation. Taken together, these results demonstrate that curcumin i...

姜黄素（curcumin）是一种具备多种生理活性的多酚类化合物。为阐明姜黄素影响系统性淀粉样变性的作用机制，我们在淀粉样载脂蛋白A-II（AApoAII）淀粉样变性小鼠模型中开展了相关研究，该模型小鼠喂食添加了姜黄素的饲料。连续12周补充姜黄素后，相较于对照组，小鼠体内的AApoAII淀粉样沉积显著增加，尤以肝脏与脾脏中最为明显。补充姜黄素的实验组小鼠的肝脏重量、血浆载脂蛋白A-II及高密度脂蛋白浓度均显著升高。RNA测序（RNA-sequence）分析显示，姜黄素摄入通过过氧化物酶体增殖物激活受体（PPAR）通路，尤其是过氧化物酶体增殖物激活受体α（PPARα）的激活，调控肝脏脂质代谢，进而提升Apoa2 mRNA的表达水平。肝脏重量的增加源于PPARα的激活与过氧化物酶体增殖。综上，本研究结果表明姜黄素……