CIL:12028, Nephrotoma, Nephrotoma suturalis, primary spermatocyte. In Cell Image Library

Time series of meiosis I, starting at metaphase, in primary spermatocytes of the crane-fly Nephrotoma suturalis that have been experimentally flattened. Time-lapse polarization microscopy using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil immersion condenser, with 2.0x zoom lens. Images captured every 15 sec over 35 min using a QImaging Retigo EXi CCD camera. Raw images were processed using 5-frame algorithm (Shribak and Oldenbourg, 2003). A movie of the series is included in this grouped set.

本数据集为经实验性扁平处理的大蚊Nephrotoma suturalis初级精母细胞内以中期为起始点的减数分裂I时期时间序列成像数据。采用搭载液晶偏振光显微镜（liquid crystal polarized light microscopy, LC-PolScope, CRi, 马萨诸塞州沃本市）的尼康Microphot SA显微镜，搭配60×/1.4 PlanApo油浸物镜、1.4数值孔径油浸聚光镜及2.0×变焦镜头开展延时偏振光显微成像；使用QImaging Retigo EXi型CCD相机，每15秒采集1幅图像，总采集时长35分钟。原始图像采用5帧算法（Shribak与Oldenbourg，2003）进行处理。本分组数据集包含该成像序列的动态影像。