C. crescentus with ParM plasmid

Tilt Series Date: 2005-06-08 Data Taken By: Gavin Murphy Species / Specimen: Caulobacter crescentus Strain: NA1000 Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 0.7°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -10.0 μm, magnification: 0x. Acquisition Software: UCSF Tomo Upload Method: rundir Processing Software Used: imod, bsoft Collaborators and Roles: R. Jensen probably gave me the ParM plasmid which was used to transform the Caulobacter, or the other Dane; Data collected and processed by Gavin Murphy Purification / Growth Conditions / Treatment: Cells were probably grown to exp. phase, then probably centrifuged. They had the ParM plasmid therein. Sample Preparation: quantifoil grid, 10nm gold, 100% humidity...

倾斜序列（Tilt Series）采集日期：2005-06-08
数据采集者：加文·墨菲（Gavin Murphy）
物种/样品：新月柄杆菌（Caulobacter crescentus）
菌株：NA1000
倾斜序列设置：单轴倾斜模式，倾斜范围为(-60.0°, 60.0°)，步长0.7°，采用恒定角增量采集，电子剂量120.0 eV/Ų，欠焦值-10.0 μm，放大倍数0x。
采集软件：UCSF Tomo
上传方式：rundir
使用的处理软件：IMOD（imod）、bsoft
合作者及分工：R·詹森（R. Jensen）或许提供了用于转化新月柄杆菌的ParM质粒，亦或是另一位丹麦籍合作者；数据采集与处理由加文·墨菲（Gavin Murphy）完成。
纯化/培养条件与处理：细胞大概率培养至指数生长期（exp. phase），随后进行离心处理，细胞中携带ParM质粒。
样品制备：Quantifoil载网（quantifoil grid），10nm金颗粒，湿度100%……