Superior Expansion of Zone 2 Hepatocytes in Pregnancy

We found that the hepatocyte proliferation wave initiated from zone 1/2 to zone 2/3 during early-to-late pregnancy, resulting in the generation of most new hepatocytes in zone 2. By single-cell RNA sequencing, we identified that Ccnd1 was highly enriched in zone 2. Mouse primary hepatocytes were isolated from 8-week-old mice by two-step collagenase perfusion, and analyzed using scRNAseq.我们发现，在妊娠早晚期，肝细胞(hepatocyte)增殖波从肝腺泡区带1/2起始并向肝腺泡区带2/3扩散，最终在肝腺泡区带2生成绝大多数新生肝细胞。通过单细胞RNA测序(single-cell RNA sequencing)，我们鉴定出Ccnd1在肝腺泡区带2呈现高富集表达。本研究通过两步胶原酶灌流法从8周龄小鼠体内分离原代肝细胞，并采用scRNAseq完成了相关分析。