Data Paper. Data Paper

File List Amphibian-skin_bacteria_16S_sequences.fna (MD5: 4cc9026d1aaa6b3f2481a3094824ae84) Amphibian-skin_bacteria_metadata.txt (MD5: 85b14afa771fd80a6de294ea9b5f991c) Description Microbial symbionts of vertebrate skin have an important function in defense of the host against pathogens. In particular, the emerging chytrid fungus Batrachochytrium dendrobatidis, causes widespread disease in amphibians but can be inhibited via secondary metabolites produced by many different skin-associated bacteria. Similarly, the fungal pathogens of terrestrial salamander eggs Mariannaea elegans and Rhizomucor variabilis are also inhibited by a variety of skin-associated bacteria. Indeed, probiotic therapy against fungal diseases is a recent approach in conservation medicine with growing experimental support. We present a comprehensive Antifungal Isolates Database of amphibian skin-associated bacteria that have been cultured, isolated, and tested for antifungal properties. At the start, this database includes nearly 2000 cultured bacterial isolates from 37 amphibian host species across 18 studies on five continents: Africa, Oceania, Europe, and North and South America. As the research community gathers information on additional isolates, the database will be updated periodically. The resulting database can serve as a conservation tool for amphibians and other organisms, and provides empirical data for comparative and bioinformatic studies. The database consists of a FASTA file containing 16S rRNA gene sequences of the bacterial isolates, and a metadata file containing information on the host species, life-stage, geographic region, and antifungal capacity and taxonomic identity of the isolate. <i>Key words</i>: <i>amphibian; antifungal; Batrachochytrium dendrobatidis; culture database; disease ecology; microbiota; probiotic therapy; skin pathogens.</i>

文件列表：Amphibian-skin_bacteria_16S_sequences.fna（MD5: 4cc9026d1aaa6b3f2481a3094824ae84）、Amphibian-skin_bacteria_metadata.txt（MD5: 85b14afa771fd80a6de294ea9b5f991c）
数据集描述：脊椎动物皮肤的微生物共生体在宿主抵御病原体侵染过程中发挥关键作用。其中，新发壶菌*Batrachochytrium dendrobatidis*（蛙壶菌）可在两栖动物中引发大规模疫病，但多种皮肤共生细菌产生的次生代谢产物可抑制其增殖。类似地，陆生蝾螈卵的真菌病原菌雅致 Mariannaea 菌（*Mariannaea elegans*）与多变根毛霉（*Rhizomucor variabilis*）同样可被多种皮肤共生细菌抑制。事实上，针对真菌病害的益生菌疗法是保护医学领域的新兴策略，且日益获得实验证据的支持。
本研究构建了一套全面的两栖动物皮肤共生细菌抗真菌分离株数据库，所有菌株均经过培养、分离及抗真菌活性检测。该数据库初始版本包含来自全球五大洲（非洲、大洋洲、欧洲、北美洲及南美洲）18项研究、37种两栖动物宿主的近2000株培养细菌分离株。随着研究社群收集到更多分离株的相关信息，本数据库将定期进行更新。
本数据库可作为两栖动物及其他生物的保护工具，同时为比较研究与生物信息学研究提供实证数据。数据库包含两类文件：一类为FASTA格式文件，存储各细菌分离株的16S rRNA基因序列；另一类为元数据（metadata）文件，包含宿主物种、生命阶段、地理区域、分离株的抗真菌能力及分类学身份等信息。
<i>关键词</i>：<i>两栖动物；抗真菌；*Batrachochytrium dendrobatidis*（蛙壶菌）；培养数据库；疾病生态学；微生物群（microbiota）；益生菌疗法；皮肤病原体。