scRNAseq_fullDataset_rdsObject

This is the RDS object containing the full dataset used to generate the Culex tarsalis midgut cell atlas (PMID: 39869679). Object consists of 12 scRNA-seq samples (allSample) that were QC'd (subset to remove ambient RNA, doublets, and cells with mitochondrial gene percentages >25%, RNA counts <100, and RNA counts >2500), log normalized individually, merged, and log normalized as one dataset (logNorm, nmn). Clusters were called using a resolution of 0.6 (res0.6). Metadata for this object has been updated to reflect cell types, timepoints, and infection condition (withMeta).

本RDS对象包含用于生成跗斑库蚊（Culex tarsalis）中肠细胞图谱（PMID: 39869679）的完整数据集。该对象包含12个经质量控制（QC）的单细胞RNA测序（single-cell RNA sequencing, scRNA-seq）样本（命名为allSample）：质控环节通过子集筛选剔除了环境RNA、双细胞污染，以及线粒体基因占比＞25%、RNA计数＜100或RNA计数＞2500的细胞；随后对所有样本分别进行对数归一化处理，合并后再以整体数据集形式完成二次对数归一化，对应数据集为logNorm与nmn。细胞聚类识别采用分辨率参数0.6（res0.6）完成。本对象的元数据已完成更新，补充了细胞类型、采样时间点及感染状态相关注释，对应元数据对象为withMeta。