This article systematically tests the contributions of eight separate molecular properties to the efficacy of bacterial translation using a formal factorial design of experiments, and reveals design p
This article investigates the impact of N-terminal sequences on translation efficiency during protein synthesis. By constructing and screening a large library of eGFP variants, the authors discovered
This article utilizes fluorescence intensity data from 1,017 cell-free buffer compositions, measured using an automated liquid handling robot and plate reader, to train machine learning models for opt
This article utilizes data from 32,376 variant sequences, which originate from the analysis of a 30-nucleotide randomized region in a fluorescent protein reporter gene. The data is applied to assess t
This article utilizes data from 1459 gene variants in Escherichia coli, including protein production levels and corresponding coding sequences, which are analyzed using a Random Forest model through m
