Additional file 1 of Amorphous SiO2 nanoparticles promote cardiac dysfunction via the opening of the mitochondrial permeability transition pore in rat heart and human cardiomyocytes

Additional file 1: Supplementary Figure 1. Perfusion for 30 min with nanoSiO2 did not cause structural alterations or inflammation in the heart apex. H&E staining of: A) untreated, B) 40 μg/mL nanoSiO2 perfusion, and C) 200 μg/mL nanoSiO2 perfusion. Supplementary Figure 2. nanoSiO2 accumulates in mitochondria from ventricle myocytes. Representative TEM micrograph of mitochondria showing swelling and its assessment by TEM-EDS from: (A) untreated rat CMs, (B) nanoSiO2 exposed rat CMs. (C) Quantification of Si content from EDS spectra.

附加文件1：补充图1。经纳米二氧化硅（nanoSiO2）灌注30分钟未引发心尖部结构改变或炎症反应。对应苏木精-伊红（H&E）染色的样本分别为：A）未处理组、B）40 μg/mL纳米二氧化硅灌注组、C）200 μg/mL纳米二氧化硅灌注组。补充图2。纳米二氧化硅（nanoSiO2）可在心室肌细胞的线粒体中蓄积。展示线粒体肿胀特征的代表性透射电子显微镜（Transmission Electron Microscopy, TEM）图像，以及采用透射电子显微镜-能谱仪（Transmission Electron Microscopy-Energy Dispersive X-ray Spectroscopy, TEM-EDS）对该特征进行的评估：（A）未处理的大鼠心肌细胞（cardiac myocytes, CMs）、（B）经纳米二氧化硅暴露处理的大鼠心肌细胞；（C）基于能谱（EDS）光谱的硅元素含量定量分析。