What miRNAs are expressed in the E10.5 and E11.5 mouse midfacial tissue?

Researching what miRNAs are expressed in the E10.5 and E11.5 mouse midfacial tissue. Methods: * E10.5: 20 dissected maxillas (.2-.25 mg w/w per maxilla; 4.5 ug of purified miRNA); 20 dissected FNP (.15-.2 mg w/w per FNP; 5 ug of purified miRNA); miRNA extracted using the Norgen miRNA Purification Kit, miRNA libraries prepared by ligation of barcoded adapters on 5' ends of small-RNA sequences, adapter was ligated to the 3' ends; miRNA-seq Illumina HiSeq * E11.5: 7 dissected maxillas (.8 mg w/w per maxilla; 3.0 ug of purified miRNA); 7 dissected FNP (1.4 mg w/w per FNP; 4.0 ug of purified miRNA); miRNA extracted using the Norgen miRNA Purification Kit, miRNA libraries prepared by ligation of barcoded adapters on 5' ends of small-RNA sequences, adapter was ligated to the 3' ends; miRNA-seq Illumina HiSeq

本研究旨在分析E10.5与E11.5小鼠面中部组织中表达的微小RNA（miRNA）。
方法：
* E10.5组：解剖获取20个上颌骨（单重0.2~0.25 mg；纯化微小RNA总量4.5 μg）、20个额鼻突（frontonasal process, FNP，单重0.15~0.2 mg；纯化微小RNA总量5 μg）；采用Norgen微小RNA纯化试剂盒提取微小RNA，通过在小RNA序列5'端连接带条形码的接头、3'端连接接头的方式构建微小RNA文库；采用Illumina HiSeq平台进行微小RNA测序。
* E11.5组：解剖获取7个上颌骨（单重0.8 mg；纯化微小RNA总量3.0 μg）、7个额鼻突（FNP，单重1.4 mg；纯化微小RNA总量4.0 μg）；采用Norgen微小RNA纯化试剂盒提取微小RNA，通过在小RNA序列5'端连接带条形码的接头、3'端连接接头的方式构建微小RNA文库；采用Illumina HiSeq平台进行微小RNA测序。