METHOD FOR INTRODUCING SUBSTANCE INTO PLANT

The present invention relates to a method for introducing a substance into a plant. The method of the present invention comprises the steps of: obtaining an enzymatically treated and isolated fertilized egg cell by (1-i) isolating a fertilized egg cell from a plant tissue containing a fertilized egg cell， and then treating the fertilized egg cell with an enzyme solution containing a plant tissue-degrading enzyme under a low-titer condition， (1-ii) treating a plant tissue containing a fertilized egg cell with an enzyme solution containing a plant tissue-degrading enzyme under a low-titer condition， and then isolating the fertilized egg cell that has been enzymatically treated， (1-iii) treating a plant tissue containing a fertilized egg cell with an enzyme solution containing a plant tissue-degrading enzyme under a low-titer condition， and simultaneously isolating the fertilized egg cell that has been enzymatically treated， (1-iv) isolating an egg cell and a sperm cell from a plant to produce a fertilized egg by fusing the cells， and then treating the fertilized egg cell with an enzyme solution containing a plant tissue-degrading enzyme under a low-titer condition， or (1-v) treating a plant tissue containing an egg cell with an enzyme solution containing a plant tissue-degrading enzyme under a low-titer condition， and then isolating the egg cell that has been enzymatically treated， and further fusing the egg cell with an isolated sperm cell; and (2) introducing a substance selected from the group consisting of nucleic acids， proteins， and peptides into the resultant enzymatically treated and isolated fertilized egg cell.

本发明涉及一种向植物导入物质的方法。本发明所述方法包含以下步骤：
(1) 通过以下任意一种方式获取经酶解处理并分离得到的植物受精卵细胞：
(1-i) 从含有受精卵细胞的植物组织中分离出受精卵细胞，随后在低浓度条件下，使用含有植物组织降解酶的酶解液对该受精卵细胞进行酶解处理；
(1-ii) 在低浓度条件下，使用含有植物组织降解酶的酶解液处理含有受精卵细胞的植物组织，之后分离得到经酶解处理的受精卵细胞；
(1-iii) 在低浓度条件下，使用含有植物组织降解酶的酶解液处理含有受精卵细胞的植物组织，同时分离得到经酶解处理的受精卵细胞；
(1-iv) 从植物中分离卵细胞与精子细胞，通过细胞融合技术制备受精卵细胞，随后在低浓度条件下，使用含有植物组织降解酶的酶解液对该受精卵细胞进行酶解处理；
(1-v) 在低浓度条件下，使用含有植物组织降解酶的酶解液处理含有卵细胞的植物组织，随后分离得到经酶解处理的卵细胞，再将该卵细胞与分离得到的精子细胞进行融合以获得受精卵细胞；
(2) 将选自核酸、蛋白质与肽类的物质导入至步骤(1)所得的经酶解处理并分离得到的植物受精卵细胞中。