Additional file 14: Table S2. of Integrative analysis of the Trypanosoma brucei gene expression cascade predicts differential regulation of mRNA processing and unusual control of ribosomal protein expression

Sheet 1: Results for coding regions present in both sheets 1 and 2 of Additional file 9: Table S1 are compared, using PC-A and BS-D for modelling. Column “BS ratio/PC ratio” is column M divided by column H and indicates possible developmental regulation of processing. Column O: “1. BS low”: mRNA amount is at least 4 times less than expected in bloodstream forms; “2. PC Low”: mRNA amount is at least 4 times less than expected in procyclic forms; 3. Both low: mRNA amount is at least 4 times less than expected in both forms; 4. Normal: remaining genes, except for “5. BS high”: mRNA amount is at least 4 times more than expected in bloodstream forms. Data for 5′-UTRs and upstream ORFs are from TritrypDB. Sheet 2: mRNAs that were at least 4 times less abundant than predicted in bloodstream forms, and more abundant than predicted procyclic forms. Sheet 3: mRNAs that were at least 4 times less abundant than predicted in both bloodstream and procyclic forms. Note that columns Q and R are not independent since each includes the mRNA abundance. Sheet 4: Statistical analyses. Sequence information was taken from from TritrypDB. (XLSX 796 kb)

工作表1：对附加文件9：表S1的工作表1与工作表2中共有的编码区结果进行比较，以PC-A与BS-D构建模型。「BS比值/PC比值」列为M列除以H列的计算结果，可提示加工过程的潜在发育调控特征。O列分类规则如下：1. 血流型低丰度（BS low）：血流型（bloodstream forms）中mRNA含量较预期值低至少4倍；2. 前循环型低丰度（PC Low）：前循环型（procyclic forms）中mRNA含量较预期值低至少4倍；3. 双低丰度：两种形态中mRNA含量均较预期值低至少4倍；4. 正常丰度：除5. 血流型高丰度（BS high）外的其余基因；5. 血流型高丰度（BS high）：血流型中mRNA含量较预期值高至少4倍。5'非翻译区（5'-UTRs）与上游开放阅读框（upstream ORFs）的相关数据均取自TritrypDB。工作表2：收录在血流型中丰度较预测值低至少4倍，且在前循环型中丰度较预测值更高的mRNA分子。工作表3：收录在血流型与前循环型中丰度均较预测值低至少4倍的mRNA分子。请注意：Q列与R列并非独立变量，因二者均包含mRNA丰度信息。工作表4：统计分析。序列数据取自TritrypDB。（文件格式为XLSX，大小796 KB）