PD-1 instructs a tumor suppressive metabolic program to restrain AP-1 activity in T cell lymphoma [ATAC-seq]

To dissect T cell lymphoma pathogenesis, we previously engineered a genetically controllable murine model of human T-NHL based on tamoxifen-inducible irreversible expression of the T-NHL oncogene ITK-SYK, which enforces strong oncogenic TCR signaling. Three days after ITK-SYK expression, we purified the oncogene-expressing cells by fluorescence-activated cell sorting (FACS) for Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq). Comparative footprint analysis of ATAC-seq data for ITK-SYK expressing T cells with and without PD-1 deletion (ex vivo and in presence of the ACLY inhibitor BMS-303141).