Heligmosomum mixtum genome sequencing

We collected H. mixtum from bank voles (Myodes glareolus) captured in Urwitalt, in northeast Poland (53° 47' 56.50"N, 21° 38' 44.4"E) in Autumn 2014. Rodents were live-trapped in wooden traps with grain and carrot or apple as bait. Each animal was weighed, sexed, measured, and sacrificed by isoflurane overdose and cervical dislocation. We carefully examined intestines under a stereoscopic microscope in search of nematodes of any species. We stored individual nematodes in 70% ethanol at –20°C until DNA extraction. For the current analysis, we used two male H. mixtum found in different host individual. We isolated DNA from individual nematodes using the Nucleospin Tissue kit (MN) or DNeasy Blood and Tissue DNA extraction kit (Qiagen).