Real-time quantitative PCR analysis of rat's brain mRNAs

We investigated the therapeutic effect of the extracellular vesicles secreted by glial progenitor cells (GPC-EV) derived from human induced pluripotent stem cell in a traumatic brain injury model. The injury was modeled using the dosed concussion to the open brain method. The male Wistar rats were performed traumatic brain injury (TBI), and after 24 hours animals were randomly divided into two groups: group 1 — rats with intranasal administration of PBS (control), and group 2 — rats with similar administration of extracellular visicles (EV) derived from glial progenitor cells. PCR analysis of brain tissues ( cortex, striatum, hippocampus) were performed on postoperative day 7 and 14 to investigate genes associated with apoptosis, and inflammation.本研究探究了人诱导多能干细胞（human induced pluripotent stem cell）来源的神经胶质祖细胞（glial progenitor cells）分泌的细胞外囊泡（extracellular vesicles, GPC-EV）在创伤性脑损伤（traumatic brain injury, TBI）模型中的治疗效果。本研究采用开放式颅脑定量冲击法构建脑震荡损伤模型，对雄性Wistar大鼠实施创伤性脑损伤造模，造模24小时后将大鼠随机分为两组：第1组为经鼻内给药磷酸盐缓冲液（PBS）的对照组，第2组为采用相同给药方式给予神经胶质祖细胞来源细胞外囊泡（EV）的实验组。分别于术后第7天和第14天对大鼠脑组织（大脑皮层、纹状体、海马体）进行聚合酶链式反应（PCR）分析，以检测与细胞凋亡及炎症反应相关的基因表达水平。