Time-series analysis of Aedes aegypti transcriptome after exposure to permethrin reveals significant upregulation of detoxifying enzymes through 24 hours post-exposure

A time series analysis after permethrin exposure was performed on pools of 5 whole Aedes aegypti adult females per replicate. Samples were collected before exposure, and 6, 10, and 24 hours after exposure to permethrin or acetone (control). After RNA extraction, samples were prepped for 3' tag-seq sequencing at the UC Davis Genome Center.

在每份重复实验中，对5只完整的埃及伊蚊成虫雌性进行了除虫菊酯暴露后的时间序列分析。在暴露前、暴露后6小时、10小时及24小时，收集了暴露于除虫菊酯或丙酮（对照组）的样本。RNA提取后，样本在加州大学戴维斯分校基因组中心进行了3'标签测序准备。