Additional file 1: Supplementary Tables. of Multiple gene movements into and out of haploid sex chromosomes

Table S1. Genes present in the male and female sex-specific region of the Ectocarpus sp. sex chromosome. Table S2. Strains used in this study, sequencing statistics and accession numbers. Table S3. Synonymous nucleotide substitutions (dS) for gametologue pairs in the brown algae included in this study. Table S4. Molecular evolutionary features and transcript levels of Ectocarpus sp. and S. japonica sex-linked genes. Table S5. PAML codeml analysis for evidence of positive selection acting on SDR genes using sequences from three Ectocarpus species and S. lomentaria. Table S6. Sex-linked genes and their autosomal homologues in Ectocarpus sp. (Esp), S. lomentaria (Slom), S. japonica (Sjap), M. pyrifera (Mpyr) and U. pinnatifida (Upin). Table S7. Orphan retrogenes in S. japonica for which the parental copy homologue is still present in Ectocarpus sp. genome. Table S8. Nucleotide and protein sequence of the orthologs of the Ectocarpus sp. male and female SDR genes recovered from E. siliculosus, E. fasciculatus, S. lomentaria, S. japonica, M. pyrifera and U. pinnatifida. Table S9. Strains of S. japonica used in this study to correlate phenotypic and genotypic sex. Table S10. Primers used to determine sex linkage in S. japonica. Table S11 Assessment of phenotypic and genotypic sex in S. japonica individuals. (XLSX 318 kb)

附表S1：水云属（Ectocarpus sp.）性染色体雌雄特异性区域所含基因。
附表S2：本研究所用菌株、测序统计数据及登录号。
附表S3：本研究涉及的褐藻性染色体同源基因对的同义核苷酸替换率（dS）。
附表S4：水云属（Ectocarpus sp.）与日本昆布（S. japonica）性连锁基因的分子进化特征及转录水平。
附表S5：基于3个水云属物种及S. lomentaria的序列，通过PAML codeml分析性决定区（SDR）基因所受正选择作用的证据。
附表S6：水云属（Ectocarpus sp.，缩写Esp）、S. lomentaria（缩写Slom）、日本昆布（S. japonica，缩写Sjap）、巨藻（Macrocystis pyrifera，缩写Mpyr）及裙带菜（Undaria pinnatifida，缩写Upin）的性连锁基因及其常染色体同源拷贝。
附表S7：日本昆布（S. japonica）中的孤儿返座基因，其亲本同源拷贝仍存在于水云属（Ectocarpus sp.）基因组中。
附表S8：从E. siliculosus、E. fasciculatus、S. lomentaria、S. japonica、M. pyrifera及U. pinnatifida中获取得到的水云属（Ectocarpus sp.）雌雄SDR基因的直系同源序列的核苷酸与蛋白序列。
附表S9：本研究中用于关联表型性别与基因型性别的日本昆布（S. japonica）菌株。
附表S10：用于鉴定日本昆布（S. japonica）性连锁的引物序列。
附表S11：日本昆布（S. japonica）个体的表型性别与基因型性别评估。（XLSX格式，文件大小318 KB）