Body size measurements collected for Acartia hudsonica during multigenerational exposure to ocean warming (OW), ocean acidification (OA), and combined ocean warming and acidification (OWA)

These data include body size measurements collected for Acartia hudsonica during multigenerational exposure to ocean warming (OW), ocean acidification (OA), and combined ocean warming and acidification (OWA) including a benign ambient condition temperature and CO2 control (AM). These data were collected every second generation between F0 and F4 for all treatments and at F11 for AM and OWA. Data were collected on C1 juveniles (C1), adult males (C6M), and adult females (C6F). Individual copepods were stained with non-acid lugol's solution, isolated in a drop of filtered seawater, and photographed using a Lumenera Infinity5-5 camera (Teledyne Lumenera, Ottawa, ON, CAN) attached to an inverted microscope (Olympus IX70, Olympus, Waltham, MA, USA) after the water droplet had been removed. Body size was measured as prosome length at C1 and C6 stages using Image-J (https://imagej.nih.gov/). The experimental environmental conditions were: 1) Ambient control (AM): 13˚C, 400 µatm CO2, pH = 8.2; 2) Ocean Acidification (OA): 13˚C, 1000 µatm CO2, pH = 7.85; 3) Ocean Warming (OW): 15°C, 400 μatm CO2, pH = 8.2; 4) Combined warming and acidification (OWA): 15°C, 1000 μatm CO2, pH = 7.85.

本数据集包含哈德逊纺锤水蚤（Acartia hudsonica）在多代暴露于海洋变暖（Ocean Warming, OW）、海洋酸化（Ocean Acidification, OA）以及海洋增温酸化复合胁迫（combined ocean warming and acidification, OWA）条件下的体长测量数据，同时设置了温和环境对照组（AM），即正常温度与CO₂浓度的基准条件。
所有处理组的样本于F0至F4代每间隔两代采样一次，AM与OWA组额外采集了F11代样本。
本次采集的样本涵盖C1期幼体（C1）、雄性成体（C6M）与雌性成体（C6F）。
单个桡足类样本经非酸性卢戈氏碘液染色后，转移至一滴过滤海水中分离固定，待水滴完全挥发后，使用搭载于倒置显微镜（Olympus IX70，奥林巴斯，美国马萨诸塞州沃尔瑟姆奥林巴斯公司）的Lumenera Infinity5-5相机（Teledyne Lumenera，加拿大安大略省渥太华Teledyne Lumenera公司）完成成像。
采用Image-J软件（https://imagej.nih.gov/）测量C1与C6期个体的体躯长作为体长指标。
实验设置的环境条件如下：1） 环境对照（AM）：13℃，400 μatm CO₂，pH=8.2；2） 海洋酸化组（OA）：13℃，1000 μatm CO₂，pH=7.85；3） 海洋增温组（OW）：15℃，400 μatm CO₂，pH=8.2；4） 增温酸化复合胁迫组（OWA）：15℃，1000 μatm CO₂，pH=7.85。