Transcriptomic changes upon overexpression of S. cerevisiae Ppz1 protein phosphatase

Overexpression of the protein phosphatase Ppz1 is toxic for yeast cells as it blocks cell cycle progression from G1 to S phase. To identify relevant changes caused by Ppz1 overexpression we have constructed a strain (ZCZ01) in wich the PPZ1 promoter has been replaced by the GAL promoter. Cell grown on raffinose were shifted to galactose and samples were taken after 30, 120 and 240 min and changes in mRNA levels analyzed by RNA-seq.蛋白磷酸酶Ppz1的过表达对酵母细胞具有毒性，因其会阻断细胞周期从G1期至S期的进程。为鉴定Ppz1过表达所诱导的相关分子变化，本研究构建了一株（ZCZ01）工程菌株，该菌株的PPZ1基因启动子已被GAL启动子（GAL promoter）替换。将在棉子糖培养基中培养的细胞转接至半乳糖培养基中，分别于30、120及240分钟时采集样本，并通过RNA测序（RNA-seq）分析mRNA（信使RNA）水平的变化。