High-throughput sequencing data of circRNAs in the gastrocnemius muscle of mice with or without right sciatic nerve denervation

This study was to identify circRNAs responsible for muscle atrophy. Skeletal muscle wasting is a common clinical feature of many chronic diseases and also occurs in response to acute events. Circular RNAs are covalently closed RNA transcripts that are involved in various physiological and pathological processes. However, the underlying mechanisms of circRNAs implicated in muscle atrophy remains unknown. Global circRNA expression profiling indicated that many circRNAs are involved in the pathophysiological processes of muscle atrophy. Overall design: The samples used for circular RNA sequencing were gastrocnemius muscle samples from denervated mice with muscle atrophy. The sequencing and analysis were conducted by OE biotech Co., Ltd. (Shanghai, China). For circular RNA sequencing, the total RNA were treated with ribo-zero kit and RNase R to remove ribosomal RNA and linear RNA, respectively. Then constructed cDNA library and qualified by the Agilent 2100 Bioanalyzer, the sequencing was performed by the Illumina sequencer.