Analysis of apoptosis in thymus.
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aMice (n = 6/group) were i.n. infected with 104 PFU of VK627, rVK627E, TsE627, rTsE627K.bMice were euthanized on 3 day p.i. and 5 day p.i., and three thymuses per group per time point were prepared for single cell suspension. Following staining with AnnexinV and PI, apoptotic cells were analyzed by flow cytometry. The early apoptotic cells were determined by annexin V+/PI− and late apoptotic cells were determined by annexin V+/PI+. The data shown represents mean ± SD for three independent experiments.cpK627E;dpE627K.
创建时间：
2015-12-02
