Expression data from mandibular arches in mouse embryos

Fusion of branchial arch derivatives is an essential event in the development of craniofacial architecture. A unique feature of the mandibular arch development is medial/lateral compartmentalization for the molecular networks. Those networks give rise to multiple region-specific organs, namely teeth, a tongue, salivary glands, and the supporting matrices such as bones and cartilages. We aimed to investigate molecular networks that govern the fusion process during mouse mandibular development. To this end, cDNA microarray technology was employed for screening of spatio-temporal gene expression in developing mandibular arch from E9.7 through E11.5. We conducted to divide a mandibular arch medially and laterally to compare both gene expression. From an embryo at E10.5, a medial (M) sample of the mandibular arch was dissected out -at just the distal end of opposed lateral lingual swellings-, and the bulk of remnant lateral region was collected as (L) sample under a stereomicroscope. Forty embryos for each time-point were used to obtain a pool of total RNA.鳃弓衍生物的融合是颅面结构发育过程中的关键事件。下颌弓发育的独有特征在于其分子网络具有内外侧区域化特性，该类分子网络可调控生成多种区域特异性器官，具体包括牙齿、舌、唾液腺以及骨、软骨等支撑基质。本研究旨在探究小鼠下颌弓发育过程中调控融合进程的分子网络。为此，本研究采用cDNA微阵列（cDNA microarray）技术，对小鼠下颌弓在E9.7至E11.5的发育阶段进行时空基因表达谱筛选。我们将下颌弓划分为内侧与外侧区域，以对比二者的基因表达差异。在E10.5的胚胎中，我们于体视显微镜下，在相对的外侧舌膨隆的远端处分离取下下颌弓的内侧（M）样本，并将剩余外侧区域的主体部分收集为外侧（L）样本。每个时间点均使用40枚胚胎以获取混合总RNA。